Cellular and thylakoid-membrane glycolipids of Chlamydomonas reinhardtii 137+.

Thylakoid membrane biogenesis in Chlamydomonas reinhardtii 137+: cell cycle variations in the synthesis and assembly of polar glycerolipid

The synthesis and assembly of thylakoid membrane polar glycerolipid (glycolipid, phospholipid, and ether lipid) have been monitored in synchronous cultures of the green alga Chlamydomonas reinhardtii 137+. A "pulse" protocol using radioactive acetate as the lipogenic precursor was devised to allow assessment of both processes during the 24-h (12-h light/12-h dark) vegetative cell cycle. Under t...

Isolation and characterization of an ether-linked homoserine lipid from the thylakoid membrane of Chlamydomonas reinhardtii 137+.

A polar diacylglycerolipid isolated from the phototrophic green alga Chlamydomonas reinhardtii 137+ (wild-type) displays chromatographic and chemical identity with the either-linked homoserine lipid 1(3),2-diacylglyceryl-(3)-O-4'(N,N,N-trimethyl)homoserine (DGTS) of the golden alga Ochromonas danica. Subcellularly, DGTS is a constituent of a fraction of thylakoid membranes purified from Chlamyd...

Thylakoid membrane biogenesis in Chlamydomonas reinhardtii 137+. II. Cell-cycle variations in the synthesis and assembly of pigment

Synthesis of the chlorophyll and the major carotenoid pigments and their assembly into thylakoid membrane have been studied throughout the 12-h light/12-h dark vegetative cell cycle of synchronous Chlamydomonas reinhardtii 137+ (wild-type). Pulse exposure of cells to radioactive acetate under conditions in which labeling accurately reflects lipogenesis, followed by cellular fractionation to pur...

Membrane lipid metabolism in Chlamydomonas reinhardtii 137+ and Y-1: I. Biochemical localization and characterization of acyltransferase activities.

The acyltransferases involved in the synthesis of the chloroplast membrane glycerolipids were analysed biochemically in dark-grown and greening Chlamydomonas reinhardtii y-1 as well as in the synchronous wild-type algae (strain 137+) and wild-type membranes. Using oleoyl-CoA as a substrate, three acyltransferase enzyme activities were detected. Glycerol-3-phosphate (glycerol-3-P) acyltransferas...

Novel chloroplast membranes are involved in protein synthesis and protein import for the biogenesis of the photosynthetic thylakoid membranes in Chlamydomonas reinhardtii